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Nucleotide vs Nucleoside – What is the Difference?

Nucleotide vs Nucleoside – What is the Difference?

These are the two terms we use frequently in molecular biology and we are expected to know it clearly. I knew the structure and assembly of both of them i.e. I knew one is with phosphate and one is without phosphate but the problem was I use to get confused every time which is what 🙁 So, as always I do, I came up with a little trick to remember which is nucleotide and which is nucleoside. Let me first show you the structure and components involved in both and at last I will tell you how I remember it.

  • Nucleotide:

  • Nucleoside:

Now let me share my trick with you guys. See in nucleotide there is t in the spelling right and it has three components so for me it is t – three, t – three, say it loud while reading it you will know what I am saying, go ahead say it out loud t – three, t – three. So for me in nucleotide t stands for three ☺️ and since we are talking about three components that means nucleotide is with phosphate. And nucleoside is of course without phosphate. Easy, isn’t it!!! Hope it helped. It might sound childish but it really helped me 🙂

Watch this video for better understanding.

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Genes vs Alleles – What is the Difference?

Genes vs Alleles – What is the Difference?

In this post we will see what are genes and alleles and the difference between them. Genes and Alleles are two terms we use so frequently in genetics. To understand these two terms we would have to start with our DNA. DNA is our genetic material and it is polymer of nucleotides which codes for all our physical characteristics, proteins etc. Here we are saying that DNA codes for all these but what exactly does that mean?

Say for example, a patch of DNA containing a particular nucleotide sequence codes for eye color. This patch of DNA is called GENE (figure a). Likewise there can be many genes in DNA which codes for various characteristics, proteins or enzymes etc.

a. gene

  • Therefore, GENE = a patch of DNA containing specific nucleotide sequence that codes for something.

Now let us go to chromosome and see the same gene on chromosome. We talked about a gene coding for eye color, just for an example assume that this gene is present on chromosome number 1. We have 23 pairs of chromosomes meaning every chromosome has a pair so here chromosome 1 also has a homologous pair. In this homologous pair also gene coding for eye color is present in the same locus as shown in figure b. This point is very important to understand that the gene location is always specific on chromosomes meaning at whichever locus the gene for eye color is present at in chromosome 1 is same for the homologous chromosomes also. This means we contain two copies of the same gene, isn’t it! And each copy in this gene is called an ALLELE. So we have two alleles of the same gene.

b. Alleles

Now let us understand one more point. See both the copies of genes are same meaning it has function of making eye pigmentation but in both the copies of the genes there can be minute difference in the nucleotide sequence which makes it code for different pigmentation or variation. For example, we saw chromosome 1 has two alleles for eye color gene, one of these alleles code for black pigmentation and the other alleles on homologous chromosome codes for brown pigmentation. And you will see phenotype of whatever alleles is dominant.

  • Therefore, ALLELE = variation of the same gene.

In a population there can be multiple alleles. That is how we have different eye colors such as black, light brown, dark brown, green, blue etc. same for different hair colors and all other physical traits that we see. But in an individual we can have only two alleles present for any particular given gene and that is because we carry homologous pairs of every chromosome. Each chromosome in that pair carries that particular allele; since we have two chromosomes in each pair we can have only two alleles of any particular gene.

Hope I am able to make it clear to you guys 🙂

 
Watch this video for better understanding.