Helping Learners Fall in Love with Biology!
All you need to know about DNA replication in prokaryotes is here. At 4:48, I have explained the OriC or origin of replication. At 17:57, I have explained leading and lagging strand. At 28:27, I have listed all the proteins and enzymes involved in replication in order. So let’s get started 🙂
DNA Replication in prokaryotes is one of the most important topics. To understand the whole basic mechanism of replication; there is this one thing I always felt while studying that would have been great if I find a list of all the enzymes involved in the process in order. So I tried and made my list which really helped me, especially during exam for quick revision. In this post I am sharing the list of enzymes involved in DNA replication of prokaryotes.
| Sr. No. | Enzyme Involved | Key Role |
| 1. | Dna A | Recognizes and binds to Ori C |
| 2. | Dna C | Loads Dna B |
| 3. | Dna B (Helicase) | Breaks the H-bonds between two DNA strands |
| 4. | SSB Protein (Single Stranded DNA Binding Protein) | Prevents ssDNA from re-forming a double helix |
| 5. | Topoisomerase II (Gyrase) | Removes topological stress |
| 6. | Dna G (Primase) | Synthesizes primers |
| 7. | DNA Pol. III | Synthesizes new strands of DNA in 5’à3’ direction |
| 8. | DNA Pol. I | Removes primers (has exonuclease activity)
Fills the gap created (has polymerase activity) |
| 9. | DNA Ligase | Seals the gap by formation of phosphodiester bond |
| 10. | Topoisomerase IV | Separates DNA catenanes |
I hope this post helps 🙂
To understand more in details about the DNA replication process in prokaryotes, watch a video on this topic here.